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BIO lab 1408 microscopy and cytology report

MICROSCOPY AND CYTOLOGY REPORT SHEET

Bio Lab 1408

1. Microscopy

Experiment (dry lab). See the powerpoint slides to complete worksheet

1.  Locate the parts below and match them to their description.

ocular lens head

objective lens stage

condenser lens with filter light

revolving nosepiece base

coarse adjustment knob rheostat

fine adjustment knob arm

iris diaphragm

1. Principles of microscopy

Experiment (dry lab)

Field of view

Note:

• power of ocular lens does not change

• to determine total magnification use: lens power X ocular lens power
• to determine diameter in mm: 40x X 4.200/total mag = diameter in mm
• to convert mm to mm: mm X 1000 = mm
• to determine area: radius is ½ of diameter. Take radius and square it then multiply by p (pi)

What happens to the field of view as magnification increases?

(It increase, decrease or stay the same.)

-Depth of focus

What happens to the depth of focus as magnification increases?

(It increase, decrease or stay the same.).

-Image orientation

Select the letter ‘e’ as you would see through the ocular if it were mounted right side up on the slide. (A, B, C or D.)

1. Cytology

Experiment (dry lab)

1. Wet Mounts
2. Eukaryotes

• Elodea leaf cells:
• Label the cell - plasma membrane, cell wall, chloroplast, and cytoplasm

• Onion cells.
• Label the cell - plasma membrane, cell wall, cytoplasm, nucleolus, and nucleus

• Human cheek cells.
• Label the cell - plasma membrane, cytoplasm and nucleus

1. Prokaryotes

Identify these bacterial cells as coccus, bacillus or spirillum

1. Cell Models: Label the animal and plant cells.

Animal cell model:

Label - centrioles (pair), cytoplasm, Golgi apparatus, mitochondria, nucleus and plasma membrane.

Nucleus:

Label - DNA (chromatin), nuclear membrane, nuclear pore and nucleolus.

Plant cell:

Label – cell wall, central vacuole, chloroplasts, cytoplasm, Golgi apparatus, plasma membrane, mitochondria and nucleus.