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BIO lab 1408 microscopy and cytology report

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MICROSCOPY AND CYTOLOGY REPORT SHEET

Bio Lab 1408

  1. Microscopy

Experiment (dry lab). See the powerpoint slides to complete worksheet

  1.  Locate the parts below and match them to their description.

ocular lens head

objective lens stage

condenser lens with filter light

revolving nosepiece base

coarse adjustment knob rheostat

fine adjustment knob arm

iris diaphragm

A

Eyepiece

ocular lens

B

Supports the ocular lenses

head

C

Holds objectives that can be rotated

revolving nosepiece

D

Connects the base and barrel

arm

E

Lens that magnifies the specimen

objective lens

F

Supports the slide

stage

G

Focuses the light onto the specimen

condenser lens with filter

H

Raises and lowers the stage for focusing

coarse adjustment knob

I

Slightly moves the stage to sharpen the image

fine adjustment knob

J

Illuminator

light

K

Light control

rheostat

L

Supports the microscope

base

M

Varies the intensity of the light

Iris diaphragm

  1. Principles of microscopy

Experiment (dry lab)

Field of view

Lens

Lens Power

Ocular lens

Total Magnification

Diameter in mm

(3 significant figures)

Diameter in mm

(3 sig figs)

Area in mm2

A = pr2

(p = 3.14)

(3 sig figs)

Scan Power

4X

10X

40X

4.200

4200

13.85

Low Power

10X

10x

100x

1.680

1680

22.16

High Power

40X

10x

400x

0.420

420

13.85

Oil Immersion

100X

10x

1000x0

0.168

168

26.73

Note:

  • power of ocular lens does not change
  • to determine total magnification use: lens power X ocular lens power
  • to determine diameter in mm: 40x X 4.200/total mag = diameter in mm
  • to convert mm to mm: mm X 1000 = mm
  • to determine area: radius is ½ of diameter. Take radius and square it then multiply by p (pi)

What happens to the field of view as magnification increases?

Decreases

(It increase, decrease or stay the same.)

-Depth of focus

What happens to the depth of focus as magnification increases?

Decreases

(It increase, decrease or stay the same.).

-Image orientation

Select the letter ‘e’ as you would see through the ocular if it were mounted right side up on the slide. (A, B, C or D.)

A

 

  1. Cytology

Experiment (dry lab)

  1. Wet Mounts
  2. Eukaryotes
  • Elodea leaf cells:
  • Label the cell - plasma membrane, cell wall, chloroplast, and cytoplasm

A

Cell wall

B

Plasma membrane

C

Chloroplast

D

Cytoplasm

  • Onion cells.
  • Label the cell - plasma membrane, cell wall, cytoplasm, nucleolus, and nucleus

A

Cytoplasm

B

Cell membrane

C

Cell wall

D

Nucleolus

E

Nucleus

 

  • Human cheek cells.
  • Label the cell - plasma membrane, cytoplasm and nucleus

A

Plasma membrane

B

Nucleus

C

Cytoplasm

  1. Prokaryotes

Identify these bacterial cells as coccus, bacillus or spirillum

A

Coccus

B

Bacillus

C

Spirillum

  1. Cell Models: Label the animal and plant cells.

Animal cell model:

Label - centrioles (pair), cytoplasm, Golgi apparatus, mitochondria, nucleus and plasma membrane.

Animal cell model #1

A

Boundary between cytosol and extracellular fluid

Plasma membrane

B

Flattened stacked discs

Centrioles (pair)

C

Synthesize ATP

Golgi apparatus

D

Cytosol + organelles

Mitochondria

E

Found in pairs, made of microtubules

Nucleus

F

Holds the DNA

Cytoplasm

Nucleus:

Label - DNA (chromatin), nuclear membrane, nuclear pore and nucleolus.

Animal cell/Plant cell Model #2

A

Double membrane

Nuclear pore

B

Assemble ribosomal subunits

Nucleolus

C

Genetic material

DNA (Chromatin)

D

Perforation on the nuclear membrane

Nuclear membrane

Plant cell:

Label – cell wall, central vacuole, chloroplasts, cytoplasm, Golgi apparatus, plasma membrane, mitochondria and nucleus.

Plant cell Model #3

A

Boundary between cytosol and cell wall

Plasma membrane

B

Synthesis of ATP

Golgi apparatus

C

Flattened stacked discs

Central vacuole

D

Supports the cell

Cell wall

E

Holds the DNA

Nucleus

F

Cytosol + organelles

Mitochondria

G

Membranous sac stores water + other substances

Cytoplasm

H

Site for photosynthesis

Chloroplasts

This problem has been solved.


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